Aqueous-GPC
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KOPTRI professionally measures the molecular weight of water-soluble polymers such as polyacrylic acid, polyethylene glycol, and chitosan hyaluronic acid, polysaccharide, etc. by utilizing Aqueous GPC (Gel Permeation Chromatography).
Water-soluble polymers are tough to analyze because they frequently absorb to the column depending on their ionic properties. Cationic polymers are particularly difficult to measure with GPC because they require various analysis techniques such as selecting specific columns depending on the ionic properties. Our institute is capable of applying suitable analysis conditions based on the polymer’s ionic (cationic, anionic, nonionic, zwitterionic) conditions. For GPC Standards, we generally use PEG/PEO and polysaccharides for GPC calibration. We also have poly(acrylic acid) and poly(styrene sulfonic acid) ready for use. If you need help with analysis, please send us a request by submitting ‘Application Form’ under ‘Contact Us’ tab, and supervising researcher will aid you with a punctual and meticulous reply.
Aqueous GPC setup
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Polymers | MW range | Temperature | Standard | Detector |
Polyethylene glycol Nonionic polymer Polyvinylpyrollidone Polyacrylic acid Polyacrylamide Poly(vinylalcohol |
600 ~ 1,000,000 Da | 40 ℃ | PEG/PEO | RI |
Cationic polymer Chitosan Protein(Peptide) |
600 ~ 1,000,000 Da | 40 ℃ | PEG/PEO | RI |
180 ~ 700,000 Da | 40 ℃ | Polysaccharide | RI | |
Hyaluronic acid Polysaccharide Oligosaccharide Carboxymethyl cellulose |
180 ~ 1,000,000 Da | 40 ℃ | Polysaccharide | RI |
* Analysis condition may vary with the ionic polymers (Cationic, anionic, and nonionic).
Aqueous GPC Chromatogram of Polysaccharide standards
Aqueous GPC Chromatogram of Polysaccharide
- Instrument : EcoSEC HLC-8320 GPC
- Column (maker, model no.): Tskgel guard PWxl+ 2 x TSKgel GMPWxl + TSKgel G2500PWxl
- Eluent : 0.1M NaNO3
- Temperature : 40 ℃
- Flow rate : 1.0 mL/min
- Injection volume, sample concentration : 100 ㎕, 3 mg/mL
- Standard : Polysaccharide
- Detector : RI
Aqueous GPC Chromatogram of standards (PEG/PEO)
Aqueous GPC Chromatogram of Collagen
- Instrument : EcoSEC HLC-8320 GPC
- Column (maker, model no.): Tskgel guard PWxl+ 2 x TSKgel GMPWxl + TSKgel G2500PWxl
- Eluent : 0.1M NaNO3
- Temperature : 40 ℃
- Flow rate : 1.0 mL/min
- Injection volume, sample concentration : 100 ㎕, 3 mg/mL
- Standard : PEG/PEO
- Detector : RI
Aqueous GPC Chromatogram of Cationic Guanidine-type polymer
- Instrument : EcoSEC HLC-8320 GPC
- Column (maker, model no.): TSKgel G5000PWXL-CP + G3000PWXL-CP (7.8 x 300mm)
- Eluent : ACN : 0.1M NaNO3 = 3 : 7
- Temperature : 40 ℃
- Flow rate : 1.0 mL/min
- Injection volumn, sample concentration : 100 ㎕, 3 mg/mL
- Standard : PEG/PEO
- Detector : RI